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Following incubation for 60 min, the reaction was terminated by adding 1% formic acid solution and the ATP and -glutamylcysteine levels were measured using RapidFire300 mass spectrometry (Agilent Technologies, Inc., Santa Clara, CA, USA) coupled with API4000 triple quadrupole mass spectrometer (AB Sciex, Framingham, MA)

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All testing is undertaken at an ISO:15189 accredited clinical laboratory