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GST Tag Interference with Protein Function Challenge : In some cases, the GST tag can interfere with the function of the target protein, particularly if the protein's active site or interaction domains are close to the fusion point

3 Ferroptosis: a novel player in depression pathogenesis Dixon and his team introduced the concept of ferroptosis in 2012, characterizing it as an iron-dependent mode of programmed cell death marked by the build-up of lipid hydroperoxides (Dixon et al., 2012)

J.BarrientosT.et al (2015)

This phenomenon resulted in glycogen accumulation and glucose uptake, which supported the pentose phosphate pathway to maintain NADPH synthesis, leading to cell survival and growth

A nonexclusive alternative explanation for the kinetic patterns with HEDS (and the decreased enzyme activity at high GSH concentrations) could be a Grx(SSCH 2 CH 2 OH) intermediate that is attacked by GSH according to monothiol mechanism (i) from Fig