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The process of necrosis activates macrophages and dendritic cells and enhances their capacity to produce nitric oxide and interferon alpha in SLE

ChIPre-ChIP U2OS-AsiSI-ER-AID cells (5 10 7 ) on 150-mm cell culture dishes were crosslinked using 1% formaldehyde for 10 min and quenched with 125 mM glycine for 2 min at room temperature