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Upon cleavage of the FRET peptide by the active enzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490 nm/520 nm

J.MayerF.SchmidB.et al (2014)

Oxidation of methionine and acetyl of the n-terminus were specified in Sequest-HT as variable modifications

Caffeic acid ameliorates colitis in association with increased Akkermansia population in the gut microbiota of mice