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Pancreatic Cancer Model Validation of shRNA Target Genes Cell lines derived from p48-Cre/p53F/+KrasL/+ (Sirt6 WT and KO) metastatic lung tumors (see above) were stably transduced with pLKO.1 vectors (Control and pooled shRNAs targeting Gstt1 ), harvested by trypsinization, washed twice in PBS, resuspended at 1 x 10 4 in 100ul PBS and injected into the venus sinus or tail vein of NOD.CB17-Prkdcscid/J mice (Jackson Laboratory) to establish lung metastases

2006 Dec

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Uri K, Bram V, Urko M M, Daniele N, Peter B, Aart M et al