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Description

Inflammatory cytokine quantification Levels of IL-6, IL-1, and TNF- in the skin, liver, and serum were quantified using ELISA kits (RUIXIN BIOTECH, China: IL-6 RX2D302196, IL-1 RX2D302066, TNF- RX2D310636) according to the manufacturers instructions 32,33

However, this outcome depends on various factors, including the availability of the substrate and whether the ROS generated by these enzymes surpass the antioxidant defense mechanisms [Frijhoff et al ., 2015]

Molecular mechanisms of hepatic lipid accumulation in non-alcoholic fatty liver disease

Research indicates that oxidative stress (OS), inflammatory responses, and lipid metabolic disturbances induced by alcohol and its metabolites are primary contributors to hepatocyte injury, positioning OS as a key target in ALD treatment