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Sheikh M, Poustchi H, Pourshams A

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For Western blot analysis, the brain hemispheres were homogenized in a RIPA lysis buffer (10 mM TrisHCl pH 7.5 containing 150 mM NaCl, 1% Nonidet P40, 0.1% SDS, 1% Triton X-100, PMSF 0.1 mg/mL) supplemented with proteinase and a phosphatase inhibitor cocktail (1:100, Sigma Aldrich)

Rothstein JD, Tabakoff B

PubMed Central , Foods , vol