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Nervonic acid has been studied for its effects on immune function in RAW 264.7 macrophages, where it has been found to activate NF-B signaling, leading to the increased production of pro-inflammatory cytokines (e.g., TNF- and IL-6) as well as enhanced nitric oxide (NO) and ROS generation in concentrations of 12.5 M or higher ( In animal models, such as in JCR rats (a model of obesity), vaccenic acid (C18:1 trans-11), a precursor to trans fats derived from ruminants, has been shown to suppress pro-inflammatory lymphocyte activity and improve the inflammatory profile of mesenteric lymphocytes ( Table 3 )

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