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The same has been observed in brain scans of those with self-reported sleep deprivation

Transfection of cells, Immunofluorescence microscopy and Subcellular fractionation of transfected cells For subcellular fractionation, HEK293T cells originating from ATCC (CRL-3216) were transfected using Ca-phosphate precipitation method

[PubMed] [Google Scholar] 36.Hosseinzadeh H, Fazly Bazzaz BS, Haghi MM

Loukas, and R.S

The observation is consistent with the reported literature, where ~70% of the thiosuccinimide conjugates are hydrolyzed via ring opening, and present thiol exchange (Supplementary Fig