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HBsAg enhances TBK1 dimerization but inhibits IRF3 activation To investigate the effect of HBs on TBK1 and IRF3 phosphorylation, THP-1 cells were transfected with HBs-encoding plasmids and then stimulated or infected with LPS, poly (I:C), and HT-DNA (Fig

Alpern RJ, Moe OW, Caplan M

doi: 10.1016/j.ccr.2004.08.031 84 QueenMMRyanREHolzerRGKeller-PeckCRJorcykCL

This colorimetric assay is based on the reaction between 5', 5'-Dithiobis 2-nitrobenzoic acid (DTNB) and GSH to form TNB, which exhibits maximum absorbance at 412 nm