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Liver sections were fixed with 2.5% glutaraldehyde and 4% paraformaldehyde, then stained with lead-uranyl acetate, and changes in mitochondrial morphology were observed with a Hitachi HT7700 electron microscope (Hitachi, Tokyo, Japan)

Gut-derived TMAO induces apoptosis by increasing intracellular mROS levels and further promoting NLRP3 assembly activation, with the underlying mechanism being the regulation of the intracellular mROS-NLRP3 axis to activate cytokinesis and release inflammatory factors, thus promoting DKD (Yi et al., 2023)

Amino acid transporter mRNA followed similar patterns

Gupta, S., Bansal, R., Sodhi, S., Brar, G., & Malhotra, M

Avoid touching the sanitized area after cleaning to reduce the risk of introducing bacteria