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However, further in vitro assays and preclinical studies with this NP-delivered metal chelator approach are warranted to improve their targeting capacity to the brain as well as to estimate the level of BBB crossing, chelation efficiency and the related toxicity before clinical applications

27 , 805815.e4 (2018)

Sperm telomere length is positively associated with the quality of early embryonic development

Cell pellets were resuspended in 0.3 ml small-scale lysis buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4, 1 mg ml 1 lysozyme, 0.75 mg ml 1 polymyxin B, 0.01 mg ml 1 DNase I) per well and incubated at 30 C, 300 rpm, 5 cm shaking diameter for 30 min