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(2020), at the beginning cells were loaded with dye, and after washing and incubation with peptides in glucose-free PBS, the efflux of dye was assessed in the supernatant (Figure 6C)

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Invasive methods that primarily rely on disruption of the BBB integrity by osmotic or biochemical means, or direct intracranial drug delivery by intracerebroventricular, intracerebral or intrathecal administration after creating reversible openings in the head are recognized [54]