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Description

The resuspended bacteria were lysed by ultrasonication on ice (four 5-min sessions with 30% amplitude, 2.0 s pulse on, 2.0 s pulse off, interspersed with 1-min pauses)

Human periosteal fibroblast explants were derived from periosteal tissue attached to bone, isolated during muco-gingival surgery, from 6 patients (3 males and 3 females)