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Statistical analyses were performed with SPSS version 13.0 software (SPSS, Inc., Chicago, IL)

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Finally, the upper layer was analyzed using the LC-MS/MS system

But with a 2mg/mL concentration (5mL of water added), that same 4mg dose requires 2.0mL of liquid, which is an entire insulin syringe worth and creates a large, uncomfortable injection volume

It is hypothesized that this increase may correspond to a rise in fat pad weight relative to overall body mass, as suggested by DEXA scans, which appear to show a rise in body fat percentage