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20 mL of this culture was supplemented with 1 mM NH 2 -Pap (from a 50 mM stock solution in 100 mM HCl) and, after 1 h, expression of the sfGFP gene (under control of the tet p/o ) was induced by adding 200 ng/mL anhydrotetracycline (aTc), followed by incubation at 30 C for 16 h

The treatment was well tolerated, as we did not observe a difference in weight between RSL3 and vehicle groups (Fig

Freeze rules: do not freeze a reconstituted (already-mixed) vial freeze-thaw degrades peptides

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In this regard, their use on one-two punch approaches (in which senescence inducers are combined with senolytics) could enhance their effectiveness, as senescent cells are more sensitive to GPX4 inhibition