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Cardiomyocytes increase the expression of AChE through the activation of muscarinic ACh receptors and ChAT gene transcription, and then synthesize ACh

The B vitamins are critical in supporting your energy, metabolism, brain function, and overall well-being

Cells were then resuspended in 500 L of fresh HBSS, strained through a 40 M cell strainer (BD Falcon, BD Biosciences, San Jose, Calif.), and analyzed using a flow cytometer (FACSCalibur or Accuri C6, BD Biosciences), which was equipped with 488-nm laser for excitation