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Stage-tips were activated with MeOH (2 60 L), washed with Buffer B (80% CH 3 CN, 20% water, 0.1% formic acid) (1 60 L), and equilibrated with Buffer A (2 60 L)

Fernndez-Checa, Liver Unit, Hospital Clnic, Centre Esther Koplowitz, Institut dInvestigacions Biomdiques August Pi i Sunyer (IDIBAPS)Centro de Investigacin Biomdica en Red de Enfermedades Hepticas y Digestivas (CIBERehd), Planta Cuarta, C/Rossell 149, Barcelona 08036, Spain e-mail: [email protected] This article was submitted to Experimental Pharmacology and Drug Discovery, a Section of the journal Frontiers in Pharmacology

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Sonication was performed with Covaris tubes which were set to 7.5% duty factor, 175 peak intensity power, and 200 cycles per burst for 120600 s

Prevention of cigarette smoke-induced lung tumors in mice by budesonide, phenethyl isothiocyanate, and N-acetylcysteine