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At harvest, phosphate-buffered saline containing NEM (10 mM) was added and the cells were then kept on ice for 10 min in lysis buffer (50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1% Triton X-100, 0.1% SDS, 1% sodium deoxycholate, protease inhibitors, and 10 mM NEM)

Three properties of acrolein make it difficult to test for mutagenicity: its high cytotoxicity, which prevents the expression of any mutagenic activity, and its high reactivity and volatility, which prevent it reaching the target sites

IDO inhibits a tryptophan sufficiency signal that stimulates mTOR: a novel IDO effector pathway targeted by D-1-methyl-tryptophan

Shefali Jariwala, best remembered as the Kaanta Laga Girl, passed away suddenly at the age of 42

The levels of anti-oxidant genes Nrf2, GpX1 and Sod1 and lipid metabolism-associated genes Srebp1c, Ppar-, and Cd36 were measured by RT-PCR