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Although the molecular role that reduced homocysteine plays in vascular disorders is unclear, experimental evidence indicates that the association between moderately elevated homocysteine levels and atherothrombotic disease is likely to be causal

The resin was washed with 15 column volumes of Wash Buffer 1 containing 20 mM HEPES pH 7.5, 100 mM NaCl, 2 mM MgCl 2 , 15 mM imidazole, 0.1% LMNG, 0.02% CHS, 10 M ligands (ghrelin or GHRP-6) and 15 column volumes of Wash Buffer 2 containing 20 mM HEPES, pH 7.5, 100 mM NaCl, 2 mM MgCl 2 , 25 mM imidazole, 0.01% LMNG, 0.005% GDN (Anatrace), 0.003% CHS, 10 M ligands (ghrelin or GHRP-6)

Bach-Faig A et al

The results were summarized in Supplementary Fig

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