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Qassim 12, 8893

In parallel, the same experiment was performed and the cell viability (metabolic activity) was determined by vital staining with neutral red dye (NR)

ATP generated through glycolysis and mitochondrial respiration provides the key signal for closure of ATP-sensitive K + channels, subsequently causing membrane depolarization and activation of voltage-dependent Ca 2+ channels (VDCCs), Ca 2+ influx and exocytosis of insulin vesicles 2