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Frozen sections of tumor tissue Firstly, EJ xenograft mice were administrated with NBD labled Gi-F-SA (8 mg/kg in 100 L PBS) and NBD labled Gi-F-CAA (8 mg/kg in 100 L PBS) by intravenous injection

6 Conclusion Over four decades since its discovery, profound insights into p53s role in cellular homeostasis and its regulatory activities have been acquired, inspiring discoveries in multiple aspects of cell biology, including genetic and epigenetic stability, cell death, senescence, various types of stress responses, metabolic regulation, and oncogenesis

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Interestingly, tryptophan (Trp) can also be used as a substrate for NAD + synthesis through the de novo pathway (Figure 1)