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To more comprehensively test how cyclin Ds interfaces are responsible for its protein interactions, we performed a series of cyclin D1-FLAG pulldown experiments, as described above, followed by mass spectrometry (see Methods)

Inhibition of both GLS1/2 and the glutaminase II pathway deserves consideration as a novel therapeutic strategy, which is still lacking in kidney research

Immunoblot analysis of cell cycle proteins Equal amounts of protein (1015 g) were boiled in sample buffer for 5 min and separated by SDS/PAGE

Thus, our findings shed light on potential new avenues for combating fungal pathogenesis

Beyond reconstituting peptides and proteins, bacteriostatic water serves several additional laboratory roles: Sample Dilution : Used for creating precise concentrations in analytical and biological assays