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For I 2 r Pf GS plasmid construct, the following forward (F) and reverse (R) primers were used: Pf GS(F): 5-GCCA GGATCC ATGAAGTCCGTGAGTTTTTCAAATAATGC-3 and I 2 Pf GS(R): 5-GCAA CTGCAG CGAAAATATGCTTGAAATGTAGAAATGATT-3 to amplify the first fragment

However, riboflavin overproduction has been achieved by obtaining mutants with overexpression of certain genes and resistance to purine analogs azaguanine, decoyinine, and methionine sulfoxide, or the riboflavin analog roseoflavin, as the B

Rosavin and tyrosol are more lipophilic and may require lipid-based formulations for optimal absorption

Additionally, all 8 A

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