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Briefly, samples were pre-treated with TE buffer and lysozyme (10 mg/mL) and incubated at 37C for 2 hours to enhance lysis of bacterial cell walls

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For example, cyst-lining epithelial cells (the primary drivers of ADPKD progression) may exhibit distinct ferroptosis vulnerabilities compared to adjacent normal tubular epithelial cells, yet the molecular basis for this difference (e.g., unique lipid metabolic profiles and antioxidant capacity) has not been rigorously characterized

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The same trend was noted for GPX-like genes (especially for GPX6 and GPX8 ) (Fig