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Abbreviations aRR: Adjusted RR BCAA: Branched-chain amino acid 1-C: Single carbon atoms FPG: Fasting plasma glucose GDM: Gestational diabetes mellitus 2 h-PG: 2 h post-load plasma glucose IADPSG: International Association of Diabetes and Pregnancy Study Groups IADPSG-GDM: GDM diagnosis based on IADPSG criteria LCMS: Liquid chromatography mass spectrometry miRNA: MicroRNA NICE: National Institute for Health and Care Excellence NICE-GDM: GDM diagnosis based on NICE criteria SES: Socioeconomic status tHcy: Total homocysteine References Saravanan P, Diabetes in Pregnancy Working Group, Maternal Medicine Clinical Study Group, Royal College of Obstetricians and Gynaecologists, UK (2020) Gestational diabetes: opportunities for improving maternal and child health

Validated assay content of 10.26 mg Purity greater than 99.8 percent Retention time and spectral identity confirmation Endotoxin levels below 0.001 EU per mg No detectable microbial contamination No detectable heavy metals High purity research compound for controlled laboratory experiments Supplied in sealed sterile research vial For research use only not for human or veterinary use For laboratory research only

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