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However, given the overlap inflammatory effects between plasma and all tissues in previous and our work, we suspected that the circulationderived proteome masked organspecific effects

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This further causes oxidative stress and inflammation in animal lungs, which is evidence of tissue damage

Thus, by lacking SIRT1 activity, ageing results in a scenario where AMPK is activated due to energy-scarce conditions, but this results in mitotic arrest through phosphorylation of p53 and increasing p16 expression (Wiley et al, 2016)