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Northern blot analysis was used to compare expression of defense-related genes in two ecotypes of A

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It was folded into a three-layered film, and the MN arrays were pressed against this parafilm laminate under 10 N force and 0.1 mm/s compression rate and held for about 30 s (to mimic manual insertion of MNs in the sclera) using TA-XT2 Texture Analyzer