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Chronic low-dose exposure to a mixture of environmental endocrine disruptors induces microRNAs/isomiRs deregulation in mouse concomitant with intratesticular estradiol reduction

Interestingly, the expression of GHR and IGF-1 were reduced in the skeletal muscle of these animals

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The separated proteins were then transferred onto a PVDF membrane (Merck Millipore) and blocked using skim milk for 2 h at room temperature

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