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Mucosal biofilms are an endoscopic feature of irritable bowel syndrome and ulcerative colitis

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The bacterial aggregates naturally settled to the bottom of the tube and the supernatants were carefully removing without disturbing the settled aggregates, then the cells were fixed in 1 mL Karnovsky fixative (2.5% glutaraldehyde, 2% formaldehyde, 0.1 M sodium cacodylate buffer, pH 7.4) and stored at 4 C until further processing