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Oncogene (2017) 36(31):443444

doi:10.1038/s41598-022-09346-y

Use a pain reliever: Ice your arm for a few minutes and take acetaminophen or ibuprofen

Successful mutants that amplified all hygromycin resistance cassette, left arm crossover region, and right arm crossover regions were plated on a fresh 7H11 plate containing 75 g/ml hygromycin