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To purify Flag-Strep ASXL1, nuclear extracts were incubated overnight at 4 C on rotator with anti-Flag M2 agarose (Sigma-Aldrich) in wash buffer, 50 mM TrisHCl, pH 7.3, 150 mM KCl, 5 mM MgCl 2 , 0.2 mM EDTA, 10% glycerol, 0.1% Tween, 2 mM -mercaptoethanol, 0.25 mM PMSF and proteases inhibitors (Sigma)

Quinone species are arguably the most renowned redox cyclers and are pivotal to many biological mechanisms 3

To view a copy of this license, visit About this article Cite this article Bai, Y., Li, K., Li, X

Neuropsychiatric disease and treatment, p

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