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At the end of treatment, the animals were euthanized, the brains surgically removed, and the two hemispheres separated

Specifically, eligible studies included participants (P) with a confirmed diagnosis of vitamin B12 deficiency, interventions (I) involving oral or sublingual vitamin B12 administration, comparators (C) using intramuscular supplementation, and outcomes (O) reporting changes in serum vitamin B12 or homocysteine levels

Serum B12 blood test MMA and homocysteine (which rise when your body is B12-starved) Complete blood count, which can reveal folate deficiency anaemia or B12-related changes Additional blood tests may be needed if results are borderline or symptoms persist