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Some of the right lungs were harvested and stored at -80C for subsequent analysis by real time quantitative polymerase chain reaction (qPCR) and western blotting, while other right lungs were perfused with cryomatrix (Thermo, New York, NY, United States) for immunofluorescence staining

Adults with small bowel disorders, low stomach acid levels or those who have had gastric surgery may not be able to absorb the B vitamin properly and are at a greater risk of deficiency

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196,197 Such studies have identified a low-frequency variant of CD300LG that is associated with fasting high-density lipoprotein cholesterol (HDL-C), 198 and a TBC1D4 variant that together are connected to higher fasting glucose levels and reduced insulin sensitivity