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The affinities of these interactions are evolutionarily fine-tuned: strong enough to maintain the kinase in an autoinhibited conformation, yet sufficiently weak to respond to diverse cellular stimuli 22

ERGIC, ER-Golgi intermediate compartments

These inhibitory auxins were thought to be held mainly by the hydrophilic amino acid residues that are located at the glutathione-binding site of the enzyme

In order to confirm whether GMP localizes in the nucleus of cultured neonatal rat cardiomyocytes (NRCMs), the cardiomyocytes were transduced with GMP using a lentiviral vector and subjected to immunocytochemical staining with anti--actin antibody (red signal in Fig