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Oto-Rhino-Laryngology 270, 13291334

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An equivalent amount of protein from each animal was mixed with 4 sample buffer with or without 2-mercaptoethanol, then separated by SDSpolyacrylamide gel electrophoresis, and transferred electrophoretically to a PVDF membrane (Merck Millipore)

In case that homoscedasticity or normality were not achieved with a logarithmic transformation, data were analyzed using a Kruskal-Wallis ranks test, followed Dunn method as a posthoc test