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The two major compounds were analysed using spectroscopic methods: 1 H and 13 C nuclear magnetic resonance (NMR) spectra were recorded in MeOH- d 4 on FT-NMR Avance III 600 MHz (Bruker, Massachusetts, USA), high-resolution electrospray ionisation mass spectrometry (HRESIMS) were obtained on Agilent 6530 Q-TOF mass spectrometer (Agilent Technologies, California, USA), Ultraviolet (UV) spectra were obtained on Shimadzu UV-2600 spectrophotometer (Shimadzu, Kyoto, Japan) and Infrared (IR) spectra were recorded on Spectrum 400 FT-IR/FT-FIR spectrophotometer (PerkinElmer, Massachusetts, USA) [29]

10.3390/molecules24213958 Molecules

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(2021) employed tandem mass tag proteomics (TMT) and mass spectrometry (MS) to analyze PBMC, identifying differential expression of proteins regulated by the liver X receptor-retinoid X receptor (LXR/RXR) pathway, which modulates lipid metabolism and inflammation, potentially influencing the expression of pro-inflammatory cytokine genes (Schulman, 2017)

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