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iPSC-derived hepatocytes as a platform for disease modeling and drug discovery

To complete our in vivo observations, we tested the effect of NO and ONOO on viability and ultrastructure of T

Geller, A

M.ToyodaJ

Antibodies The following antibodies were used: anti-TRIP12 (Proteintech, 25303, 1:1,000), antip-I-kB (Cell Signaling Technology, 9246 s, 1:1,000), antip-IKK (Cell Signaling Technology, 2078 s, 1:1,000), anti-I-B (Cell Signaling Technology, 4812 s, 1:1,000), antip-p65 (Cell Signaling Technology, 3033, 1:1,000), anti-HA (Cell Signaling Technology, 3724 s, 1:1,000), anti-FLAG (SIGMA, 7425, 1:1,000, or Cell Signaling Technology, 14793 s, 1:1,000), anti-TRAF2 (Abcam, ab-126758, 1:1,000), anti-OTUD5 (Cell Signaling Technology, 20087, 1:1,000), anti-ARMC7 (NOVUS, NBP1-82129, 1:1,000), anti-ZGPAT (Proteintech, 23203, 1:1,000), anti-GTF2B (Proteintech, 16467, 1:1,000), anti-MEAF6 (Themo Fisher Scientific, PA5-40704, 1:1,000), anti-ZBED1 (Proteintech, 26140, 1:1,000), anti-PRDM2 (Proteintech, 27718, 1:1,000), anti-NR2F2 (COUP-TFII) (Cell Signaling Technology, 6434 s, 1:1,000), anti-DHX32 (Proteintech, 68306-1-lg, 1:1,000), anti-Rad18 (Cell Signaling Technology, 9040 s, 1:1,000), anti-Actin (Santa Cruz Biotechnology, sc-47778, 1:1,000), anti-ubiquitin (P4D1, Santa Cruz Biotechnology, sc-8017, 1:1,000), anti-OTUD3 (Proteintech, 29622, 1:1000), anti-OTUD4 (Proteintech, 25070, 1:1000), anti-UBR5 (Santa Cruz Biotechnology, sc-515494, 1:1000), and anti-K48ubiquitin (Abcam, ab-140601, 1:1,000)