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FIGURE 8 3.8 Immunohistochemical findings The immunohistochemical investigation demonstrated a significant increase in expression of proapoptotic makers, Bax, and caspase-3, in MTX treated group, which was associated with decreased antiapoptotic marker, Bcl-2, expression when compared to the CTRL group (Figure 9)

This imbalance sustains a self-amplifying loop of oxidative stress, neuroinflammation, and mitochondrial dysfunction, reinforcing chronic pain pathophysiology

[2] [5] Multifunctional enzymes also offer advantages in terms of the regulation of their synthesis

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