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Chen J-L, Luo C, Pu D, Zhang G-Q, Zhao Y-X, Sun Y, et al

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After yeast cells lysis ( A ) and ( B ), Ras activation was determined using the GST-RBD(Byr2) fusion protein, which binds with high affinity to the GTP-associated form of Ras

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Immunohistochemistry of Ki67 used VECTASTAIN-ABC (Vector Laboratories) with 40 objective image capture (Olympus EX51 Microscope)