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Before NMR analysis, the dry aqueous extracts were suspended in 650 L of 100 mM sodium phosphate buffer (pH 7.4, in D 2 O containing 0.25% 3-(trimethylsilyl)-propionic-2,2,3,3-d4 acid (TSP) for chemical shift referencing) and transferred into 5 mm NMR tubes

By ensuring adequate levels of this essential nutrient, individuals can support healthy sleep patterns, improve energy levels, and promote better appetite control