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In U937 and HepG2 cells, blocking GSH efflux prevented puromycin-induced apoptosis (Ghibelli et al, 1998), suggesting depletion of GSH played an important role in initiating apoptosis in some cell types

Importantly, FOXO4-DRI showed no significant toxicity towards normal TM3 Leydig cells, demonstrating its selective action against senescent cells

The peptide is typically administered via subcutaneous injection

In summary, future therapeutic innovations should move beyond simple immune suppression to a more integrated model that addresses oxidative stress, adaptive immunity, and innate immune amplification simultaneously

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