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Anwar A et al

!WARNING : since rat hemoglobin crystallizes in hypoosmotic buffers, for cell lysis and desalting buffer a 20 mM Tris base solution and a 20 mM Tris base solution containing 100 mM NaCl are used, respectively

doi: 10.3390/ijms232315309

Another route is via condensation of the glutarimide ring unit with the 3-nitrophthalic anhydride, with consequent reduction of the nitro group to an amine group, thus producing pomalidomide 64