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PMC Cognitive Function and Neurological Research Robillard JM, et al

Cells were cultured for another 20 h after FBS supplementation and viewed with a fluorescence microscope (DMi8, Leica, Germany)

qRTPCR analyses were performed with cDNA and gene-specific oligonucleotides with a LightCycler 480 SYBR Green I Master Kit (Roche Diagnostics GmbH) using a LightCycler 480 II machine