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The collected blood samples were centrifuged for 10 min at 4000 rpm and the plasma was separated and stored at 80 C for the subsequent analysis

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3.4 Mitochondrial Transport Once glutamine is taken up into the cell, it needs to be transported into the mitochondria for further metabolism, particularly in tissues with high energy demands

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Even though the results that ammonium ferric citrate (FAC)-treated chondrocytes showed increased apoptosis and MMP3 and MMP13 level links iron overload to chondrocyte apoptosis (42), it may shed light on the role of chondrocyte ferroptosis in OA