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10.1093/nar/gky896 34 KarstenS

10.1177/0960327119895811 67 LuH.WuL.LiuL.RuanQ.ZhangX.HongW.et al (2018)

Sections were blocked in PBS with 10% donkey serum (DS) and 0.1% Triton X-100 for 2 h at room temperature, incubated with primary antibodies diluted in PBS with 3% donkey serum and 0.1% Triton X-100 overnight at 4 C and subsequently washed and incubated with secondary antibodies diluted in 0.05% BSA in PBS and DAPI for 2 h at room temperature