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The protein samples were digested by adding Trypsin (Thermo Scientific) at a 1:20 ratio (w/w) and incubated at 37C overnight

Mechanistically, the authors reported that the uremic toxin, indoxyl sulfate, activates the aryl hydrocarbon receptor (AhR), and experiments using AhR knockout mice confirmed that AhR activation is a key driver of BBB damage (Bobot et al., 2020)

While an association between thin endometrium and age has not yet been established, this study points to a possible link between the two entities