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After 24 h, cells were collected to determine firefly and renilla luciferase activities using the Dual-Glo luciferase assay system kit (Promega Inc, Madison, WI, USA) in a Tecan Infinite M200 Microplate Reader (Tecan Austria GmbH, A-5082 Grdig, Austria)

(5) Standard viability assays, including those that quantify cell density and metabolic activity, indicated that tested cultures supported higher fibroblast numbers compared to controls