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The NIH milk thistle report found, Results from clinical trials of milk thistle for liver diseases have been conflicting or havent been clinically meaningful

This imbalance disrupts mitochondrial TCA activity and OXPHOS, leading to decreased intracellular ATP levels in immature DCs[73, 74]

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The comparative threshold cycle (Ct) method was used to measure the relative expression, where 2 Ct represents the fold change in expression, as previously described ( TABLE 1 Primer sequences used in qRT-PCR

Holbrook KA, Byers PH