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However, T166A or N169A single mutation did not change the activation (Fig

These plasmids were digested with EcoRI or NcoI, and transformed into appropriate S

Modulating ACSL4/GPX4 and FSP1 axes presents a promising therapeutic strategy for oxalate-induced AKI

Two mechanisms of reaction of these species with DNA and proteins are known: type I mechanism, involving one-electron transfer, and type II mechanism which can be divided into two subtypesmajor mechanism based on reaction with DNA via singlet oxygen, resulting in formation of 8-oxoguanine, and minor mechanism based on formation of a superoxide anion radical which undergoes disproportionation reaction, giving hydrogen peroxide and reactive oxygen species (IARC 2012)